End-to-end bulk RNA-seq orchestrator - takes raw FASTQ reads through QC and trimming (FastQC, fastp/Trim Galore), alignment and quantification (STAR, Salmon, featureCounts), assembles a gene-level counts matrix, then …
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name: bulk-rnaseq
description: End-to-end bulk RNA-seq orchestrator — takes raw FASTQ reads through QC and trimming (FastQC, fastp/Trim Galore), alignment and quantification (STAR, Salmon, featureCounts), assembles a gene-level counts matrix, then hands off to differential expression (pydeseq2), pathway/GSEA enrichment (pathway-enrichment), and publication figures (scientific-visualization). Use whenever the user has bulk RNA-seq reads or quant output and wants a complete, reproducible differential-expression workflow — e.g. "analyze my RNA-seq", "FASTQ to DESeq2", "run nf-core/rnaseq", "STAR/Salmon quantification", "build a counts matrix for DESeq2", or "go from reads to differentially expressed genes and enriched pathways". Routes between an nf-core/rnaseq (Nextflow) path and a standalone STAR/Salmon path, and covers experimental design, strandedness, and QC gates. For single-cell RNA-seq use the scanpy skill instead.
license: MIT
metadata: {"version": "1.0", "skill-author": "K-Dense Inc."}
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# Bulk RNA-seq
## Overview
This skill orchestrates a complete, **defensible** bulk RNA-seq differential-expression study, from raw sequencing reads to enriched pathways and figures. It is a router, not a reimplementation: most stages already have dedicated skills in this repo, and this skill connects them in the right order, fills the one real gap (raw reads → a gene-level counts matrix), and enforces the design and QC decisions that determine whether the final result is trustworthy.
"Defensible" means three things, applied throughout:
- **Reproducible** — pinned pipeline/tool versions, containers where possible, recorded parameters, fixed random seeds.
- **Quality-gated** — QC is inspected and acted on before, during, and after quantification, not skipped.
- **Statistically sound** — adequate replication, a design that matches the biology, counts handled correctly, and FDR-controlled testing.
The pipeline is: **FastQC/trim → align/quant (STAR/Salmon) → counts → DE (pydeseq2) → enrichment (pathway-enrichment) → figures**.
## When to Use This Skill
Use this skill when the user wants to:
- Go from FASTQ files (or a sequencing run) to differentially expressed genes and pathways.
- Run or configure `nf-core/rnaseq`, or align/quantify with STAR, Salmon, or featureCounts.… load the full skill through Skill Me